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John C Alverdy University of Chicago Division of the Biological Sciences, Chicago, Illinois, USA

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from endogenous sources (i.e. those from pathogens within the patient’s own microbiome) are responsible for a given SSI, remains unknown. While there is little doubt that intraoperative contamination from local environmental sources can certainly lead

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Paige Buffington Department of Physician Assistant Studies, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Alexia M Sebghati Department of Physician Assistant Studies, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Kasey B Stewart Department of Physician Assistant Studies, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Samantha Lawson Department of Physician Assistant Studies, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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Oleg Karaduta Department of Physician Assistant Studies, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

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on the mode of delivery is a proposed underlying etiology for the sequela experienced by individuals born by cesarean section. When an infant is born vaginally, there is vertical transmission of the vaginal and rectal microbiome to the infant, with

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Elizabeth A Coler Department of Chemistry, University of Connecticut, Storrs, Connecticut, USA

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Wanxuan Chen Department of Chemistry, University of Connecticut, Storrs, Connecticut, USA

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Alexey V Melnik Department of Chemistry, University of Connecticut, Storrs, Connecticut, USA
Arome Science Inc., Farmington, Connecticut, USA

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James T Morton Gutz Analytics LLC, Boulder, Colorado, USA

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Alexander A Aksenov Department of Chemistry, University of Connecticut, Storrs, Connecticut, USA
Arome Science Inc., Farmington, Connecticut, USA

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, metabolomics has experienced a significant surge in interest, largely due to its growing role in analyzing the effect and function of the microbiome. The gut microbiome in particular, comprising a multitude of different bacterial, fungal and archeal species, is

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Morgan A Maly Center for Conservation Genomics, Smithsonian National Zoo and Conservation Biology Institute, Washington, DC, USA
Department of Animal Care Science, Smithsonian National Zoo and Conservation Biology Institute, Front Royal, Virginia, USA
Department of Biological Sciences, College of Sciences, North Carolina State University, Raleigh, North Carolina, USA
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

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Adrienne E Crosier Department of Animal Care Science, Smithsonian National Zoo and Conservation Biology Institute, Front Royal, Virginia, USA

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Mia M Keady Center for Conservation Genomics, Smithsonian National Zoo and Conservation Biology Institute, Washington, DC, USA
Nelson Institute for Environmental Studies, University of Wisconsin-Madison, Madison, Wisconsin, USA

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Reade B Roberts Department of Biological Sciences, College of Sciences, North Carolina State University, Raleigh, North Carolina, USA

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Matthew Breen Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

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Carly R Muletz-Wolz Center for Conservation Genomics, Smithsonian National Zoo and Conservation Biology Institute, Washington, DC, USA

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Introduction The role of the gut microbiome in animal health has been of increasing interest in wildlife medicine ( Sugden et al. 2020 , Gillman et al. 2022 ) and conservation ( Redford et al. 2012 , Bahrndorff et al. 2016

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Victoria Poplaski Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA

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Faith Sawyer Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA

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Sarah E Blutt Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA

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, Greicius & Virshup 2019 ). Recently, the human microbiome has emerged as another source of factors that regulate the function of ISCs. The advent of the Human Microbiome Project resulted in a wealth of information regarding the putative microbial

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Hanh KD Nguyen School of Geography, Planning and Spatial Sciences, University of Tasmania, Sandy Bay, Tasmania, Australia
Healthy Landscapes Research Group, University of Tasmania, Sandy Bay, Tasmania, Australia

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Penelope J Jones Healthy Landscapes Research Group, University of Tasmania, Sandy Bay, Tasmania, Australia
Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia

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Dave Kendal Healthy Landscapes Research Group, University of Tasmania, Sandy Bay, Tasmania, Australia
Future in Nature Pty Ltd, Australia

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Shane M Powell Tasmanian Institute of Agriculture (TIA), University of Tasmania, Sandy Bay, Tasmania, Australia

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Emily J Flies School of Geography, Planning and Spatial Sciences, University of Tasmania, Sandy Bay, Tasmania, Australia
Healthy Landscapes Research Group, University of Tasmania, Sandy Bay, Tasmania, Australia

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environment. In humans, microbiomes directly impact host health in many ways through immune and brain function, as well as energy storage and use ( Clemente et al. 2012 , Nicholson et al. 2012 ). Presumably, microbiomes have similarly strong influences on

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Simran Kaur Cheema School of Biological Sciences, Institute for Global Food Security, Queen’s University Belfast, UK

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Ranran Li School of Biological Sciences, Institute for Global Food Security, Queen’s University Belfast, UK

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Simon J S Cameron School of Biological Sciences, Institute for Global Food Security, Queen’s University Belfast, UK

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. Emerging evidence suggests that human milk contains a dynamic microbiota, which helps infants to colonise the gut microbiome development, trains and matures the immune system and prevents colonisation by potential pathogens ( Fernandez et al. 2013 , Ruiz

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Nadja Paeslack Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Johannes Gutenberg-University Mainz, Germany

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Christoph Reinhardt Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Johannes Gutenberg-University Mainz, Germany
German Center for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Mainz, Germany

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, Turnbaugh et al. 2007 , Neish 2009 , Thursby & Juge 2017 ). The entity of microbial genes present in this ecosystem is known as the microbiome ( Lederberg & McCray 2001 , Hooper et al. 2002 , Qin et al. 2010 ). Residing in the gut lumen, the

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Andrija Karačić Department of General Surgery, University Hospital Sveti Duh, Zagreb, Croatia
The Gut Microbiome Center (CCM), Zagreb, Croatia

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Jasna Novak Department of Biochemical Engineering, University of Zagreb Faculty of Food Technology and Biotechnology Faculty, Zagreb, Croatia

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Alan Ivković Department of Orthopaedics and Traumatology, University Hospital Sveti Duh, Zagreb, Croatia
School of Medicine, University of Zagreb, Zagreb, Croatia
Department of Clinical Medicine, University of Applied Health Sciences, Zagreb, Croatia

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Introduction Omics technological breakthroughs contributed to the field of intestinal microbiome analysis over the past decade ( Ng et al. 2023 ). The intestinal microbiome is a complex microbial community residing in the gastrointestinal

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Anil Sakamuri Vascular Biology Center and Department of Medicine, Medical College of Georgia at Augusta University, Augusta, Georgia, USA

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Pritam Bardhan UT Microbiome Consortium, Department of Physiology & Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA

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Ramakumar Tummala UT Microbiome Consortium, Department of Physiology & Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA

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Franck Mauvais-Jarvis Section of Endocrinology and Metabolism, Tulane University Health Sciences Center, New Orleans, Louisiana, USA

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Tao Yang UT Microbiome Consortium, Department of Physiology & Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA

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Bina Joe UT Microbiome Consortium, Department of Physiology & Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA

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Benard Ojwang Ogola Vascular Biology Center and Department of Medicine, Medical College of Georgia at Augusta University, Augusta, Georgia, USA

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bedding and allowed time to roam the cage and deposit fecal matter at will. Two pellets were collected and frozen immediately for 16S rRNA gene sequencing. These frozen samples were shipped to the University of Toledo Microbiome Core for further processing

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